Cspa induction temperature
WebThe mechanism of the CspA derepression may be a stabilization of the chromosomal cspA transcripts under the growth inhibition condition, similar to that of the cold-shock induction of CspA. To this end, we have … WebThe induction of CspA is mainly due to an increase in mRNA stability. Its half-life is 12 s at 37°C, but between 15 min and 30 min at 15°C in E. coli (Tanabe et al., 1992; Jiang et al., 1993; Fang et al., 1997; Gualerzi et al., 2003). If the coding region of cspA is fused to the constitutive promoter lpp, it is still cold inducible. This ...
Cspa induction temperature
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WebThe Escherichia coli cspA gene, encoding the major cold-shock protein CspA, was deprived of its natural promoter and placed in an expression vector under the control of the … WebApr 1, 1999 · CspI induction at lower temperatures. CspA, CspB, CspG, and CspI are all cold shock inducible. However, upon cold. shock, the temperature dependence of CspA induction is broad-
WebJan 15, 2010 · Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its transcript, whose secondary structure at … WebJan 15, 2010 · Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its …
WebMar 15, 1999 · As with cspA mRNA, the cold induction of CspA also depended on the initial level of this protein in cells at different stages of growth and the maximum increment (30-fold) was seen in mid-to-late log cells . In all cases, however, the maximum level attained by CspA after cold shock (≈2% of total soluble protein) is such that the net … WebJul 1, 1999 · 1 Introduction. When exponentially growing Escherichia coli cells are shifted from 37°C to low temperature, cells stop growing as the synthesis of most cellular proteins is either shut off or significantly reduced. This growth lag period is termed the acclimation phase, during which cold-shock proteins such as CspA , CspB , CspG and CspI are …
WebMar 1, 2002 · Among them, CspA is the major cold shock protein because of its highest induction (more than 10% of the total cellular protein synthesis) after temperature …
WebFollowing temperature downshift, CspA, CspB, CspG and CspI are highly induced, as indicated by the arrows in Fig. 2C (Wang et al. 1999). However, interestingly, the … little einsteins great sky race gameWebJul 20, 2024 · The authors regarded CspB as being much more resistant to kanamycin than to chloramphenicol in comparison to the other two MCSPs and attributed this result to … little einsteins game boy advanceWebJun 3, 2024 · The optimal short-induction temperature was investigated in a range between 15°C and 37°C for 30 min before increasing the temperature to 37°C for 24 h to confirm that the high PHB productivity resulting in this study is a result of the cold-shock cspA promoter and that 15°C is the optimal induction temperature. little einsteins gets grounded for nothingWebBoth temperature cycling between 15 and 25 degreesC and stepwise temperature downshifts between 37, 29, 21, and 13 degreesC led to multiple inductions of the cspA … little einsteins ice cream trainWebIt was further found that CspA induction can be achieved not only by culture dilution but also by the simple addition of nutrients, and that it was almost completely abolished in … little einsteins good knight and bad knightWebSep 5, 2008 · For all single-temperature downshift experiments, however, the promoter became repressed 60–120 min after initiation of cooling. Both temperature cycling between 15 and 25 °C and stepwise temperature downshifts between 37, 29, 21, and 13 °C led … little einsteins halloween thomasWebThe 5′ end of the cspA mRNA contains a regulatory sequence (cold box), which stabilizes the mRNA at low temperature, enabling cold shock induction (Xia et al., 2002). The … little einsteins going on a trip